Analysis of co-translationally acting factors regulating the biogenesis of chloroplast-encoded proteins
We found that heat acclimation leads to remodelling of the synthesis of specific chloroplast-encoded photosys-tem II core proteins. Furthermore, we revealed a broad network of ribosome-associated factors in chloroplasts, containing many putative modulators and integrators with altered ribosome-binding behaviour during heat acclima-tion. Based on these findings, the proposed project aims to deepen the understanding how protein biogenesis is regulated during temperature acclimation. We plan to investigate selected putative modulators for their contribu-tion for tuning translation itself and to examine central steps of co-translational nascent polypeptide processing and membrane integration.