TRR 175 The Green Hub - Central Coordinator of Acclimation in Plants
print


Breadcrumb Navigation


Content

C02

Dissection of the plastidic unfolded protein response in Chlamydomonas reinhardtii

In this project we aim at characterizing the chloroplast UPR (cpUPR) using Chlamydomonas as a model system. For this we will first trigger a cpUPR by inducing the expression of unfolded proteins in the chloroplast and quantify the cpUPR in a time-resolved manner by MS-based proteomics, tran-scriptomics, and metabolomics. Second, we will identify mutants defective in the cpUPR by forward and reverse genetics approaches. Third, we will functionally characterize the chloroplast universal stress protein A, a high-potential integrator of chloroplast acclimation responses. Fourth, we will sys-tem-wide monitor acclimation responses of photoautotrophically grown Chlamydomonas cells to heat and cold.