TRR 175 The Green Hub - Central Coordinator of Acclimation in Plants

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Analysis of co-translationally acting factors regulating the biogenesis of chloroplast-encoded proteins

Our overall goal is to understand how chloroplast protein biogenesis is precisely modulated to secure proteome homeostasis during acclimation in Chlamydomonas reinhardtii. Ribosome profiling and quantitative proteomics serve for in-depth analysis of the dynamics of chloroplast translation and ribosomal complexes during changing environmental conditions. These global studies will be com-plemented by the functional characterization of specific co-and post-translationally acting factors, such as molecular chaperones, that exhibit central roles for protein maturation and subcellular sorting.